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Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus

I Woclawek-Potocka, Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
J Komiyama, Faculty of Agriculture, Okayama University, Okayama , Japan
J Saulnier-Blache, Institut Louis Bugnard, INSERM U317, Université Paul Sabatier, Toulouse, France
E Brzezicka, Department of Reproductive Immunology, Polish Academy of Sciences, Olsztyn, Poland
M Bah, Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
K Okuda, Faculty of Agriculture, Okayama University, Okayama , Japan
D Skarzynski, Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland

Correspondence: Izabela Woclawek-Potocka, Email: iza77{at}o2.pl

Abstract

Lysophosphatidic acid (LPA) modulates prostaglandin (PG) synthesis via LPA receptor type 3 (LPA3) in the murine endometrium. The lack of functional LPA3 in mice may lead to embryo mortality. In the present study, we examined the role of LPA in the bovine uterus. We confirmed that LPA is locally produced and released from the bovine endometrium. Moreover, there are enzymes involved in LPA synthesis (phospholipase (PL) D2 and PLA2) in the bovine endometrium during estrous cycle and early pregnancy. Expression of the receptor for LPA (LPA1) was positively correlated with the expression of PGE2 synthase (PGES) and negatively correlated with the expression of PGF2 synthase (aldose reductase with 20 alpha-hydroxysteroid dehydrogenase activity - PGFS) during early pregnancy. In vivo LPA induced P4 and PGE2 secretion was inhibited by LPA1 antagonist (Ki16425). The overall results indicate that LPA is locally produced and released from the bovine endometrium. Moreover, LPA1 gene expression in the endometrium during the estrous cycle and early pregnancy indicates that LPA may play autocrine and/or paracine roles in the bovine uterus. LPA1 gene expression is positively correlated with the expression of the enzyme responsible for luteotropic PGE2 production (PGES) in endometrium. In the cow, LPA stimulates P4 and PGE2 secretion. Thus, LPA in the bovine reproductive tract may indirectly (via endometrium) or directly support CL action via the increase of P4 synthesis and the increase of PGE2/ PGF2 ratio. It suggests that LPA may serve as an important factor in the maintenance of early pregnancy in cow.