This Article Accepted Manuscript (PDF) Erratum All Versions of this Article: 136/3/361    most recent REP-08-0171v1 Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Donofrio, G. Articles by Capocefalo, A. PubMed PubMed Citation Articles by Donofrio, G. Articles by Capocefalo, A.

Bacterial infection of endometrial stromal cells influences bovine herpesvirus 4 immediate early gene activation: a new insight into bacterial and viral interaction for uterine disease.

G Donofrio, Salute Animale, Sezione di Malattie Infettive degli Animali, University of Parma, Facoltà di Medicina Veterinaria, Parma, 43100, Italy
M Sheldon, Department of Veterinary Clinical Sciences, University of London, London, United Kingdom
L Ravaneti, Dipartimento di Salute Animale, Università di Parma, Parma, Italy
S Cavirani, Dipartimento di Salute Animale, Università di Parma, Parma, Italy
S Herath, Department of Veterinary Clinical Sciences, University of London, London, United Kingdom
A Capocefalo, Dipartimento di Salute Animale, Università di Parma, Parma, Italy

Correspondence: Gaetano Donofrio, Email: gaetano.donofrio{at}unipr.it

Abstract

Abstract

Experimental infection with the gammaherpesvirus Bovine herpesvirus 4 (BoHV-4) rarely establishes disease, yet BoHV-4 is commonly associated with uterine disease in cattle. Uterine disease involves co-infection with bacteria such as Escherichia coli, which stimulate the production of prostaglandin E2 (PGE2) by endometrial cells. BoHV-4 replication depends on Immediate Early 2 (IE2) gene transactivation, and in the present study, PGE2, E. coli or its lipopolysaccharide (LPS), up-regulated the IE2 gene promoter in uterine cells. Bacterial co-infection is important for BoHV-4 uterine disease.