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INTERACTION OF OESTRADIOL AND NAFOXIDINE HYDROCHLORIDE WITH PROGESTERONE IN DECIDUOMATA FORMATION

Summary.: The effect of nafoxidine HCl and oestradiol on progesterone and oestradiol uptake by rat deciduomata was studied. Rats were ovariectomized and their uteri traumatized; they were subsequently injected for 4 days with 1·5 mg of progesterone and either oestradiol (0·005, 0·05, 0·1, 0·5 or 1·0 µg), or oestradiol (0·05 µg) and nafoxidine HCl (0·5, 5, 50, 500, 1000µg). Tritiated oestradiol or progesterone was injected 4 hr before killing the rats on the last day of treatment.

Uterine horns (traumatized and contralateral non-traumatized cornua) were removed and immediately prepared for the determination of radio-activity. Total non-volatile radio-activity and the radio-activity associated with the protein, ether-soluble and water-soluble fractions were determined. Ether soluble fractions were chromatographed on silica gel; areas associated with major steroid components were isolated and counted for radio-activity.

Both nafoxidine HCl and oestradiol effectively suppress the deciduomata response. The effects of each compound on the above parameters are not comparable in all cases. These differences, and their significance, are discussed.