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PREVENTION OF COAGULATION IN MONKEY SEMEN BY SURGERY

Monkey semen characteristically coagulates during ejaculation, and samples obtained by electrical stimulation consist wholly or largely of a rubbery mass. Some spontaneous dissolution occurs when the ejaculate is held in vitro at room or body temperature, but more than half the mass, usually about 75%, remains unchanged. Use of monkey semen for laboratory studies or for artificial insemination is hampered by this feature. To some extent, the trouble can be remedied by the use of proteolytic enzymes, of which trypsin seems to be the least deleterious to sperm viability (Roussel & Austin, 1967), but the presence of trypsin raises problems for many biochemical studies. Accordingly, the possibility was investigated that surgical removal of the coagulum-producing gland from the male tract might result in the ejaculation of coagulum-free semen, without seriously interfering with the volume and other semen charac