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DEEP-FREEZING OF JACKASS AND STALLION SEMEN IN CONCENTRATED PELLET FORM

Since Polge & Rowson (1952) devised their technique of preserving bull semen at ultra-low temperatures, several attempts have been made to apply this preservation method to stallion semen.

Early reports by Szumowski (1954) and Iljinskaja (1956) were encouraging but (so far as it is known to us) later experiments conducted by Barker & Gandier (1957), Zmurin (1959, 1960), Buell (1963), Schäfer & Baum (1964), Polge & Minotakis (1964) and others failed to make further progress.

However, recently, Nagase & Graham (1964) reported an entirely new method of freezing bull semen in a pelleted form. At the same time Polge & Minotakis (1964) found a better survival rate in jackass semen than in stallion semen, using a conventional freezing method. In conjunction with our experimental work on the seasonal influence of light on sperm production