| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Articles |
Cloned mice typically display abnormal development, such as overgrowth of fetuses and placentae. Quantitative expression analysis of eight imprinted genes (H19, Igf2, Igf2r, Air, Peg1/Mest, Peg3, Nuronatin (Nnat) and Ndn) and an alternate transcript of Igf2 (P0) in embryonic stem cloned fetuses and placentae at days 9.5, 12.5 and 17.5 after mating was carried out by real time PCR to investigate whether epigenetic modification of imprinted genes is responsible for overgrowth of the fetus and placental hypertrophy. In addition, the methylation pattern through the bisulphite sequencing method in differentially methylated regions of H19 and Igf2r was examined in day 9.5 fetuses and placentae. The results showed clearly that the expression of H19 gene decreased in cloned fetuses at days 12.5 and 17.5 after mating and in placentae at day 17.5 after mating, and Igf2 was also repressed in fetuses at days 9.5 and 12.5 after mating and in placentae at day 17.5 after mating. In contrast, the transcription of P0, which is a placental-specific transcript variant of Igf2, increased at more than four times the control in cloned placenta at day 12.5 after mating. Day 9.5 fetuses that have developed normally revealed only hypermethylated alleles in the H19 differently methylated region (DMR), and both hyper- and hypomethylated alleles in the Igf2r DMR2. These results show that inappropriate reprogramming in some imprinted genes affects the development of cloned embryos, and that aberrant P0 Igf2 transcription in particular may cause the overgrowth of cloned fetuses and placentae.
This article has been cited by other articles:
 |
|
 |
|
  Y. Jincho, Y. Sotomaru, M. Kawahara, Y. Ono, H. Ogawa, Y. Obata, and T. Kono Identification of Genes Aberrantly Expressed in Mouse Embryonic Stem Cell-Cloned Blastocysts Biol Reprod, April 1, 2008; 78(4): 568 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-P. Kim, A. Thurston, C. Mummery, D. Ward-van Oostwaard, H. Priddle, C. Allegrucci, C. Denning, and L. Young Gene-specific vulnerability to imprinting variability in human embryonic stem cell lines Genome Res., December 1, 2007; 17(12): 1731 - 1742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Iwahashi, H. Yoshioka, E. W Low, J. R McCarrey, R. Yanagimachi, and Y. Yamazaki Autonomous Regulation of Sex-Specific Developmental Programming in Mouse Fetal Germ Cells Biol Reprod, October 1, 2007; 77(4): 697 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Shimozawa, Y. Sotomaru, N. Eguchi, S. Suzuki, K. Hioki, T. Usui, T. Kono, and M. Ito Phenotypic abnormalities observed in aged cloned mice from embryonic stem cells after long-term maintenance. Reproduction, September 1, 2006; 132(3): 435 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Schulz, T. R. Menheniott, K. Woodfine, A. J. Wood, J. D. Choi, and R. J. Oakey Chromosome-wide identification of novel imprinted genes using microarrays and uniparental disomies Nucleic Acids Res., July 19, 2006; 34(12): e88 - e88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Constant, M. Guillomot, Y. Heyman, X. Vignon, P. Laigre, J.L. Servely, J.P. Renard, and P. Chavatte-Palmer Large Offspring or Large Placenta Syndrome? Morphometric Analysis of Late Gestation Bovine Placentomes from Somatic Nuclear Transfer Pregnancies Complicated by Hydrallantois Biol Reprod, July 1, 2006; 75(1): 122 - 130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Feil, M. Lane, C. T. Roberts, R. L. Kelley, L. J. Edwards, J. G. Thompson, and K. L. Kind Effect of culturing mouse embryos under different oxygen concentrations on subsequent fetal and placental development J. Physiol., April 1, 2006; 572(1): 87 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. W. Sun, A. C. Yang, Y. Feng, Y. J. Sun, Y. f. Zhu, Y. Zhang, H. Jiang, C. L. Li, F. R. Gao, Z. H. Zhang, et al. Temporal and parental-specific expression of imprinted genes in a newly derived Chinese human embryonic stem cell line and embryoid bodies Hum. Mol. Genet., January 1, 2006; 15(1): 65 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Smith, R. E. Everts, X. C. Tian, F. Du, L.-Y. Sung, S. L. Rodriguez-Zas, B.-S. Jeong, J.-P. Renard, H. A. Lewin, and X. Yang Global gene expression profiles reveal significant nuclear reprogramming by the blastocyst stage after cloning PNAS, December 6, 2005; 102(49): 17582 - 17587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Niwa, R. Takano, Y. Obata, H. Hiura, J. Komiyama, H. Ogawa, and T. Kono Nuclei of Oocytes Derived from Mouse Parthenogenetic Embryos Are Competent to Support Development to Term Biol Reprod, November 1, 2004; 71(5): 1560 - 1567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Weidman, S. K. Murphy, C. M. Nolan, F. S. Dietrich, and R. L. Jirtle Phylogenetic Footprint Analysis of IGF2 in Extant Mammals Genome Res., September 1, 2004; 14(9): 1726 - 1732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. Dindot, P. W. Farin, C. E. Farin, J. Romano, S. Walker, C. Long, and J. A. Piedrahita Epigenetic and Genomic Imprinting Analysis in Nuclear Transfer Derived Bos gaurus/Bos taurus Hybrid Fetuses Biol Reprod, August 1, 2004; 71(2): 470 - 478. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
